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facs sheath fluid (Eppendorf AG)

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Eppendorf AG facs sheath fluid

Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. The low biomass was obtained through dilution of the high biomass mock standard (10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction) and after (diluted <t>post-extraction)</t> <t>DNA</t> extraction and through sorting of the mock standard into 10 5 cells mL -1 and 10 5 noise events mL -1 using a non-selective SYBR® Green I staining. Dilution and sorting were performed in triplicate. <t>FACS</t> sheath fluid and aPBS diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

Facs Sheath Fluid, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 96/100, based on 609 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+sheath+fluid/%C3%97+FACS/bio_rxiv__2025__11__13__688239-171-12-18
Average 96 stars, based on 609 article reviews

facs sheath fluid - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities"

Article Title: An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities

Journal: bioRxiv

doi: 10.1101/2025.11.13.688239

Figure Legend Snippet: Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. The low biomass was obtained through dilution of the high biomass mock standard (10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction) and after (diluted post-extraction) DNA extraction and through sorting of the mock standard into 10 5 cells mL -1 and 10 5 noise events mL -1 using a non-selective SYBR® Green I staining. Dilution and sorting were performed in triplicate. FACS sheath fluid and aPBS diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

Techniques Used: Sequencing, Extraction, DNA Extraction, SYBR Green Assay, Staining

The removal of relic DNA during sorting resulted in discrepancies between the non-sorted and sorted samples. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. The low biomass was obtained through dilution of the high biomass mock standard (10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction) and after (diluted post-extraction) DNA extraction and through sorting of the mock standard into 10 5 cells mL -1 and 10 5 noise events mL -1 using a non-selective SYBR® Green I staining. Dilution and sorting were performed in triplicate. FACS sheath fluid and aPBS diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

Figure Legend Snippet: The removal of relic DNA during sorting resulted in discrepancies between the non-sorted and sorted samples. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. The low biomass was obtained through dilution of the high biomass mock standard (10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction) and after (diluted post-extraction) DNA extraction and through sorting of the mock standard into 10 5 cells mL -1 and 10 5 noise events mL -1 using a non-selective SYBR® Green I staining. Dilution and sorting were performed in triplicate. FACS sheath fluid and aPBS diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

Techniques Used: Sequencing, Extraction, DNA Extraction, SYBR Green Assay, Staining

Low biomass samples obtained through dilution of the high biomass mock standard (orange; 10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction; turquoise) and after (diluted post-extraction; green) DNA extraction and sorted noise events (10 5 mL -1 ; purple) did not display an improved clustering. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. Dilution and sorting with a non-selective SYBR® Green I cell staining were performed in triplicate. FACS sheath fluid (light grey) and aPBS (brown) diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

Figure Legend Snippet: Low biomass samples obtained through dilution of the high biomass mock standard (orange; 10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction; turquoise) and after (diluted post-extraction; green) DNA extraction and sorted noise events (10 5 mL -1 ; purple) did not display an improved clustering. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. Dilution and sorting with a non-selective SYBR® Green I cell staining were performed in triplicate. FACS sheath fluid (light grey) and aPBS (brown) diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

Techniques Used: Extraction, DNA Extraction, Sequencing, SYBR Green Assay, Staining

Benzonase nuclease activity selectively depleted relic DNA of Veillonella atypica , Pseudomonas aeruginosa ASV63, Escherichia - Shigella coli ASV 11/42/43/77/88/179 and Salmonella enterica ASV 25/201. AMPure XP clean-up eliminated relic DNA but was not selective resulting in a distorted community composition. A decrease in the (relic) DNA concentration prior to sorting resulted in an inflation in absolute and relative contaminant sequencing read counts attesting the need of in silico data decontamination. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. Cells were sorted using a non-selective SYBR® Green I staining. A FACS sheath fluid blank was included to identify and track the FACSome contaminants introduced during FACS-sequencing.

Figure Legend Snippet: Benzonase nuclease activity selectively depleted relic DNA of Veillonella atypica , Pseudomonas aeruginosa ASV63, Escherichia - Shigella coli ASV 11/42/43/77/88/179 and Salmonella enterica ASV 25/201. AMPure XP clean-up eliminated relic DNA but was not selective resulting in a distorted community composition. A decrease in the (relic) DNA concentration prior to sorting resulted in an inflation in absolute and relative contaminant sequencing read counts attesting the need of in silico data decontamination. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. Cells were sorted using a non-selective SYBR® Green I staining. A FACS sheath fluid blank was included to identify and track the FACSome contaminants introduced during FACS-sequencing.

Techniques Used: Activity Assay, Concentration Assay, Sequencing, In Silico, SYBR Green Assay, Staining

FACS:

Article Title: Shape-based separation of synthetic microparticles
Article Snippet: 1Materials Department, University of California, Santa Barbara, CA, USA.. 2Department of Electrical Engineering, Stanford University, Stanford, CA, USA.. 3Institute for Collaborative Biotechnologies, University of California, Santa Barbara, CA, USA.

Article Title: An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities
Article Snippet: Heat-killed samples (30 minutes at 96 °C), aPBS and 0.2 μm-filtered (Merck, Darmstadt, Germany) samples were included to inform the gating process that distinguished intact from damaged microbial cell subpopulations and cell-free noise. .. The sorted cell fraction consisted of 288,000 cells collected in 1 mL FACS sheath fluid in 1.5 mL Eppendorf® DNA LoBind collection tubes (Merck, Darmstadt, Germany). ..

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Journal: bioRxiv

Article Title: An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities

doi: 10.1101/2025.11.13.688239

Figure Lengend Snippet: Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. The low biomass was obtained through dilution of the high biomass mock standard (10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction) and after (diluted post-extraction) DNA extraction and through sorting of the mock standard into 10 5 cells mL -1 and 10 5 noise events mL -1 using a non-selective SYBR® Green I staining. Dilution and sorting were performed in triplicate. FACS sheath fluid and aPBS diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

Article Snippet: The sorted cell fraction consisted of 288,000 cells collected in 1 mL FACS sheath fluid in 1.5 mL Eppendorf® DNA LoBind collection tubes (Merck, Darmstadt, Germany).

Techniques: Sequencing, Extraction, DNA Extraction, SYBR Green Assay, Staining

Journal: bioRxiv

Article Title: An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities

doi: 10.1101/2025.11.13.688239

Figure Lengend Snippet: The removal of relic DNA during sorting resulted in discrepancies between the non-sorted and sorted samples. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. The low biomass was obtained through dilution of the high biomass mock standard (10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction) and after (diluted post-extraction) DNA extraction and through sorting of the mock standard into 10 5 cells mL -1 and 10 5 noise events mL -1 using a non-selective SYBR® Green I staining. Dilution and sorting were performed in triplicate. FACS sheath fluid and aPBS diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

Article Snippet: The sorted cell fraction consisted of 288,000 cells collected in 1 mL FACS sheath fluid in 1.5 mL Eppendorf® DNA LoBind collection tubes (Merck, Darmstadt, Germany).

Techniques: Sequencing, Extraction, DNA Extraction, SYBR Green Assay, Staining

Journal: bioRxiv

Article Title: An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities

doi: 10.1101/2025.11.13.688239

Figure Lengend Snippet: Low biomass samples obtained through dilution of the high biomass mock standard (orange; 10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction; turquoise) and after (diluted post-extraction; green) DNA extraction and sorted noise events (10 5 mL -1 ; purple) did not display an improved clustering. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. Dilution and sorting with a non-selective SYBR® Green I cell staining were performed in triplicate. FACS sheath fluid (light grey) and aPBS (brown) diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

Article Snippet: The sorted cell fraction consisted of 288,000 cells collected in 1 mL FACS sheath fluid in 1.5 mL Eppendorf® DNA LoBind collection tubes (Merck, Darmstadt, Germany).

Techniques: Extraction, DNA Extraction, Sequencing, SYBR Green Assay, Staining

Journal: bioRxiv

Article Title: An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities

doi: 10.1101/2025.11.13.688239

Figure Lengend Snippet: Benzonase nuclease activity selectively depleted relic DNA of Veillonella atypica , Pseudomonas aeruginosa ASV63, Escherichia - Shigella coli ASV 11/42/43/77/88/179 and Salmonella enterica ASV 25/201. AMPure XP clean-up eliminated relic DNA but was not selective resulting in a distorted community composition. A decrease in the (relic) DNA concentration prior to sorting resulted in an inflation in absolute and relative contaminant sequencing read counts attesting the need of in silico data decontamination. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. Cells were sorted using a non-selective SYBR® Green I staining. A FACS sheath fluid blank was included to identify and track the FACSome contaminants introduced during FACS-sequencing.

Article Snippet: The sorted cell fraction consisted of 288,000 cells collected in 1 mL FACS sheath fluid in 1.5 mL Eppendorf® DNA LoBind collection tubes (Merck, Darmstadt, Germany).

Techniques: Activity Assay, Concentration Assay, Sequencing, In Silico, SYBR Green Assay, Staining

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facs sheath fluid (Eppendorf AG)

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1) Product Images from "An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities"

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